Endoplasmic reticulum chaperone BiP regulates ER stress and cell fate of endometrial stromal cells during the decidualization program

L FERNÁNDEZ, C KONG, M TRYFONOS, P,J. BRIGHTON, T RAWLINGS, S GORI,, C PÉREZ LEIRÓS, E LUCAS, JJ. BROSENS AND R RAMHORST.

Maceio

Congreso; X SLIMP (LatinAmerican Society for Maternal-fetal Interface and Placenta; 2024

LatinAmerican Society for Maternal-fetal Interface and Placenta

Endoplasmic reticulum chaperone BiP regulates ER stress and cell fate of endometrial stromal cells during the decidualization program.Laura Fernández1,2, Chow-Seng Kong2, Maria Tryfonos2, Paul J. Brighton2, Thomas M. Rawlings2, Maria Soledad Gori1, Claudia Pérez Leirós1, Emma S. Lucas2, Jan J. Brosens2 and Rosanna Ramhorst11 CONICET, Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales IQUIBICEN, Buenos Aires2 Warwick Medical School, Division of Biomedical Sciences, University of Warwick, Coventry, United Kingdom.Objective: During the secretory phase of the menstrual cycle, endometrial stromal cells (EnSCs) undergo decidual differentiation. The decidualization program involves secretory changes that trigger endoplasmic reticulum (ER) stress. Additionally, decidualization leads to emergence of two subpopulations of stromal cells: mature decidual and senescent decidual cells. Both ER stress and senescence contribute to the production of key implantation mediators, and their dysregulation has been implicated in early reproductive failures. The aim of this work is to identify ER stress associated genes that might control endometrial senescence during decidualization.Methods: Expression of ER stress associated genes was in silico evaluated using scRNAseq data (GSE127918). Primary culture of EnSC were established from endometrial biopsies. Cells were transfected with specific HSPA5 siRNA and in vitro decidualized. Gene expression, protein production and secretion were tested by RT-qPCR, WB and ELISA, respectively. Senescence-associated β-galactosidase (SA-β-Gal) activity was determined by fluorescence assay. *p