Sexual dimorphism in the immunometabolic profile hofhofbauer cells.

D PAPARINI, E GRASSO, F AGUILERA, B LARA, M. A ARSLANIAN, V LELLA, V HAUK, F MERECH, C SCHUSTER, S GORI, C MELLER, M MARTÍ, R RAMHORST, D VOTA AND C PÉREZ LEIRÓS

San LUis

Congreso; Reunión Anual de la Sociedad Argentina de inmunología (SAI); 2023

Sociedad Argentina de inmunología (SAI)

104 (37) SEXUAL DIMORPHISM IN THE IMMUNOMETABOLIC PROFILEOFHOFBAUER CELLSDaniel Paparini1, Esteban Grasso1, Franco Aguilera1,2, Brenda Lara1, M. AgustinaArslanian3, Victoria Lella3, Vanesa Hauk1, Fátima Merech1, Claudio Schuster2, SoledadGori1, Cesar Meller3, Marcelo Martí2, Rosanna Ramhorst1, Daiana Vota1 & ClaudiaPérezLeirós11Immunopharmacology lab. Department of Biological Chemistry, School of Sciences, Universityof Buenos Aires. IQUIBICEN-CONICET. Ciudad Universitaria, Pab. 2, (1428) Buenos Aires,Argentina. 2Bioinformatic Lab. Department of Biological Chemistry, School of Sciences, Universityof Buenos Aires. IQUIBICEN-CONICET. Ciudad Universitaria, Pab. 2, (1428) Buenos Aires,Argentina. 3Obstetric Service, Hospital Italiano, Buenos Aires,Argentinadaniel.paparini@gmail.comAims: Hofbauer cells (HB) are the only foetal immune cell population within thestroma of healthy placenta. However, the functional properties of these cells arepoorly described. HB are transcriptional and chromosomically similar to yolk sacmacrophages. Here we studied the effect of sexual dimorphism in theimmunometabolism of human placental HBat term.Methodology: HB cells were obtained from placental tissue by enzymaticdigestion from healthy pregnant women at term along with autologous PBMCs.HB were cultured overnight and the phenotypic profile, efferocytosis, glucoseuptake and lipid droplets accumulation were evaluated by flow cytometry andELISA. We have started with bioinformatic analysis in public database tounderstand the differential imprinting of placentas with male or female newborns.Results: HB from female placenta (fHB) present higher production ofantiinflammatory markers CD163, CD206 and CD39 accompanied by lessersecretion of IL-1 than HB from male placentas (mHB) (*P