Prevalence of Fasciola hepatica infection in Galba cousini and Galba schirazensis from an Andean region of Ecuador

CELI-ERAZO, MARITZA; ALDA, PILAR; MONTENEGRO-FRANCO, MARÍA; PAVON, DIEGO; MINDA-ALUISA, ELIZABETH; CALVOPIÑA, MANUEL; POINTIER, JEAN PIERRE; HURTREZ-BOUSSÈS, SYLVIE; CEVALLOS, WILLIAM; BENÍTEZ-ORTÍZ, WASHINGTON; RODRÍGUEZ-HIDALGO, RICHAR

Veterinary Parasitology: Regional Studies and Reports

Elsevier

Año: 2020 vol. 20

2405-9390

Some Lymnaeid snails are intermediate hosts of the liver !uke Fasciola hepatica, the causal agent of fasciolosis, a zoonotic parasitic disease. Human and livestock fasciolosis has been reported in a highland community located in the Chimborazo Province of the Ecuadorian Andes. However, no previous study has been carried out to identify which snail species act as intermediate host/s of F. hepatica. This study "rst aimed to identify the intermediate snail species and secondly to determine the prevalence of natural infection with F. hepatica in 230 lymnaeid snails sampled from irrigation and drainage canals in this area. The "rst objective entailed observa- tions of shell morphology and internal organs as well as sequencing of the cytochrome oxidase subunit 1 (COI) gene. For the second objective, we used classic parasitological methods (observation of rediae and cercarial emission) and PCR ampli"cation specie-speci"c to F. hepatica. COI haplotype networks were built to elucidate phylogeographic relationships between the snail populations from this highland community with other American and worldwide populations. We identi"ed two lymnaeid Galba cousini and Galba schirazensis and found high infection rates of F. hepatica in G. cousini, but these di#ered according to the method used, with PCR showing a higher rate (61 ± 20%) compared to rediae observation (29 ± 17%). F. hepatica in G. schirazensis was identi"ed only by DNA ampli"cation. G. cousini populations were genetically structured by geographic distance whereas G. schirazensis populations showed very low genetic diversity. The higher abundance and infection rate of G. cousini compared to G. schirazensis suggests that the former is likely the specie responsible for F. hepatica transmission in this region.