PERSONAL DE APOYO
MAROTTE Clarisa
artículos
Título:
Low protein intake magnifies detrimental effects of ovariectomy and vitamin D on bone.
Autor/es:
CLARISA MAROTTE; MACARENA MS GONZALES CHAVES; GRETEL G PELLEGRINI; SILVIA M FRIEDMAN; FIMA LIFSHITZ; PATRICIA MANDALUNIS; SUSANA N ZENI
Revista:
CALCIFIED TISSUE INTERNATIONAL
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 93 p. 184 - 192
ISSN:
0171-967X
Resumen:
Protein-induced changes in bone and calcium homeostasis could potentially be greater in the elderly and in women at risk for osteoporosis. We hypothesize that a low protein intake would magnify the negative changes in bone metabolism seen in vitamin D (vitD) insufficiency and/or estrogen deficiency. The present study was undertaken to better understand how a low protein diet along with vitD insufficiency could affect bone metabolism using a rodent ovariectomized (OVX) model. Rats (n = 60) underwent ovariectomy (OVX) or sham operation. The first 15 days after surgery, all ratswere fed a standard rodent diet. Thereafter, rats (n = 10/group) were fed a low protein diet (LP; 2.5 %) or a control diet (NP; 12.5 %) with 100 IU% vitD (?D; cholecalciferol) or without vitD (-D) for 45 days. The groups were as follows: SHAM ? NP ? D (control); SHAM ? LP ? D; SHAM ? LP - D; OVX ? NP ? D; OVX ? LP ? D; OVX ? LP - D. Body weight (BW) of control and OVX ? NP ? Dgroups increasedwhile those feeding the LP diet, independently of vitD feedings, decreased (p�.05). The OVX ? LP - D group presented the lowest serum Ca, phosphorus and osteocalcin levels and the highest CTX levels (p�.05).At the end of the study, total skeleton bonemineral content, proximal tibia bone mineral density, bone volume and trabecular number levels decreased as follows: SHAM ? NP ? D (controls)[SHAM ? LP ? D[OVX ? NP ? D[SHAM ? LP - D[OVX ? LP ? D[OVX ? LP - D (p�.05). A low protein diet negatively affected bone mass and magnified the detrimental effects of vitD and/or estrogen deficiencies. Keywords Bisphosphonate Bone Low protein intake Rats Vitamin D insufficiency