Antibiofilm properties of lactic acid bacteria and their metabolites against Salmonella enterica serotype Enteritidis on eggshell surface

MERINO, LINA E.; LLANA, MARIÁNGELES NOTO; GARÓFALO, AILÍN; TREJO, FERNANDO M.; MARINA A GOLOWCZYC

Current Research in Microbial Sciences

ELSEVIER

Año: 2025 vol. 8

2666-5174

Salmonella enterica serotype Enteritidis (SE) is a pathogenic bacterium commonlyfound on the surface of eggshells. In this study, we investigated the biofilm formation ofa specific strain of Salmonella enterica serotype Enteritidis, CIDCA 115 (SE 115) oneggshells. Additionally, we examined the impact of Lentilactobacillus kefiri strains83113 and 8321, as well as Lactiplantibacillus plantarum 83114, and their metabolitespresent in the supernatant on the biofilm formation of SE 115. Scanning electronmicroscopy revealed that SE 115 formed a mature biofilm structure on the eggshell.During co-incubation and pre-incubation, lactic acid bacteria strains significantlyreduced the formation of SE 115 biofilm (p < 0.05 and p < 0.01, respectively) comparedto SE 115 grown alone. The cell-free supernatants of lactic acid bacteria also exhibiteda reduction in SE 115 biofilm formation and modified its structure. Co-incubation withSE 115 induced alterations in the composition of biofilm matrix components, notably inthe levels of fimbria curli and cellulose. The qPCR analysis revealed that, after 48hours of incubation, the expression of the csgD gene, a critical regulator of biofilmformation, remained unchanged compared to planktonic cells. However, genesassociated with the production of biofilm matrix components, curli (csgA) and cellulose(bcsA), exhibited heightened expression in the presence of lactic acid bacteriacompared to the planktonic state. This study highlights the potential of lactic acidbacteria strains and their metabolites as innovative strategies formanaging Salmonella biofilm formation in the context of poultry production.