INVESTIGADORES
MARTINEZ CALEJMAN Camila
congresos y reuniones científicas
Título:
Respuesta suprarrenal por CLP en animales Insulinorresistentes
Autor/es:
CALDARERI, L.; SCHIAPPACASSE, A; WISZNIEWSKI, MORENA; MARTINEZ CALEJMAN C; ESTEBAN MARTIN REPETTO; CYMERYNG C. B.
Lugar:
Rosario
Reunión:
Congreso; Reunion Anual de la Sociedad Argentina de Diabetes; 2022
Institución organizadora:
Sociedad Argentina de Diabetes
Resumen:
IntroductionSepsis is characterized by a disproportionate response to infection that results in systemic inflammation and can lead to multi-organ dysfunction and death. Under these conditions, the proper functioning of the stress response system and the production of glucocorticoids (GCs), is critical. Previously, we demonstrated the gradual establishment of adrenocortical insufficiency a model of insulin resistance in rats fed 30% sucrose in drinking water (SRD).ObjectiveTo evaluate the production of CGs in animals of the SRD group in a model of sepsis by ligation surgery and cecal puncture (CLP).Materials and MethodsAdult male Wistar rats on a standard diet or with SRD for 12 weeks underwent CLP or sham surgery (SHAM) and were euthanized 24 hours later. Metabolic determinations were performed in blood and tissues, GCs (RIA), and immunohistochemistry, western blot and RT-PCR for other parameters.ResultsAnimals with CLP showed higher levels of corticosteronemia than those corresponding to sham surgery, although the relative increase was smaller in the DRS group. Significantly higher levels of plasma ACTH were only observed in the SRD-CLP group (Table 1).C-SHAMC-CLPSRD-SHAMSRD-CLPCORTICOSTERONE (ng/ml)51.88 ± 16.5176.6 ± 14.2 a141.5 ± 29.8198.2 ± 39.57bPLASMA ACTH (pg/ml)129.3 ± 8.7121.3 ± 18.1108.2 ± 18.2333.1 ± 101.4bap