INVESTIGADORES
MARTINEZ CALEJMAN Camila
congresos y reuniones científicas
Título:
Short-term intermittent cold induces browning of white adipose tissue and increases glucose clearance
Autor/es:
PALOMA BIVORT HAIEK; BARBARA ABRIL VALENTE; ESTEBAN MARTIN REPETTO; CYMERYNG, CORA B.; MARTINEZ CALEJMAN C
Lugar:
Mar del Plata
Reunión:
Congreso; Reunion Anual de Sociedades de Biociencias; 2022
Institución organizadora:
Sociedad Argentina de Investigaciones Clinicas
Resumen:
Introduction: Adipocytes are typically classified as being white or brown. White adipocytes (WA), which are the most abundant, store energy in a single large lipid droplet. Brown adipocytes (BA) have multiple lipid droplets, many more mitochondria, and specialize in burning energy to generate heat through non-shivering thermogenesis, which requires the expression of Uncoupling Protein 1 (UCP1). In response to cold environments, the sympathetic nervous system stimulates thermogenesis to BAT utilize large amounts of glucose, fatty acids, and other nutrients to fuel heat production. Notably, some WA can also adopt the metabolic characteristics of classic BA under certain conditions in a process called browning. These socalled brown-in-white (brite) adipocytes mainly appear in subcutaneous fat depots express Ucp1, and can also contribute to whole body energy expenditure. The energy expenditure properties of brown orbeige adipocytes is garnering interest in developing strategies to increase BA number and/or activity to treat obesity and other metabolically related diseases. Aim: To study the effect of a short-term intermittent cold (IC) protocol on the activity of BAT and the browning capacity of WAT of mice. Materials & methods: We subjected 8 week-old male C57bl 6 mice to increasing short-term periods (5-15 min/5 days a week) at 4ºC for a month IC, and we measured its effect on BAT and WAT and in serum parameters. Results & Conclusions: Although there were no significant changes in BAT morphology as well as UCP1 expression, WAT histology showed a marked reduction in lipid droplet size and a higher expression of UCP1 in the IC group. Serum glucose levels were markedly reduced in the fed state (156±2 mg/dL vs 210±12 mg/dL p