?Novel antimicrobial peptides from the skin secretion of the Patagonian specie Pleurodema thaul (Anura Leptodactylidae: Leiuperinae)?

NATALIA L. CANCELARICH; ALYNE RODRIGUES DE ARAUJO; ALEXANDRA PLACIDO; CARLA SOUSA; PÉREZ, LUIS ORLANDO; BASSO, NÉSTOR GUILLERMO; EATON, PETER ; LEITE, JOSÉ R.S.A. ; MARANI, MARIELA M.

Paranaíba

Simposio; III Simposio Latino Americano de Biotecnología de Biotecnología de Nordeste- Encuentro Luso Brasilero de Biotecnología; 2015

Antimicrobial peptides (AMPs) have proven to be effective against a broad spectrum of pathogens and several have been approved for clinical use. These are obtained from various natural sources, being the skin of amphibians one of the most abundant sources. An important feature of AMPs is it versatility given by a great diversity chain lengths, net charge, hydrophobicity and spectrum of action. They come from different families, genera and species. In this sense the Patagonian region has a broad biodiversity with more than 60 species of unexplored amphibians, so it is a promising source of new peptides.The objective of this work was the identification and characterization of skin AMPs from Patagonian frog Pleurodema thaul by mRNA isolation, cDNA cloning and sequencing.