Interleukin 17 (IL-17) is involved in some effects of Murine Hepatitis Virus (MHV-A59)

JOSÉ L. APARICIO; MAITE DUHALDE-VEGA; UYTTENHOVE, CATHERINE; VAN SNICK, JACQUES; COUTELIER, JEAN-PAUL; LILIA A. RETEGUI

Congreso; LXIII Annual Meeting of the Argentinean Society for Immunology, IV Meeting of LASID (Latin American Society for Immunodeficiencies) and the Second French-Argentinean Immunology Congress; 2015

Background: MHV-A59 triggers various mouse pathologies such as hepatitis, thymus involution and hypergammaglobulinaemia. We reported that viral infection induced autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH) and release of uric acid and high-mobility group box protein 1. Since IL-17 is a pro-inflammatory cytokine involved in autoimmune diseases, cancers and viral infections, we explored the effects of neutralizing IL-17 with a monoclonal antibody (MAb MM17F3) on MHV activity.Methods: C57BL/6 mice were infected with MHV-A59 (104 TCID50) and 4, 7 and 11days post-infection were inoculated with 150 μg of anti IL-17 MAb (MM17F3). After infection, serum transaminases and uric acid concentration were determined enzymatically, immunoglobulins, IL-17 and IL-6 were measured by sandwich ELISA, and autoAb to FAH were detected by Western-blot. Liver histopathology was also explored.Results: Treatment of animals with the neutralizing MAb MM17F3 decreased the level of transaminases (P