Nanoscale distribution of surface proteins in Trypanosoma cruzi

ESCALANTE, GONZALO; LOPEZ, LUCIA FERNANDA; SZALAI, ALAN M.; MUCCI, JUAN; PUIG PARADA, RAQUEL; CAMPETELLA, OSCAR; STEFANI, FERNANDO D.

Rio de Janeiro

Congreso; SUPERRESOLUTION CONGRESS 2024 IN LATIN AMERICA; 2024

Trypanosoma cruzi (T. cruzi), the parasite responsible for Chagas disease, evades the host's immune system by coating its outer membrane with sialic acid (SA). T. cruzi cannot synthesize SA. It has a specific membrane–bound enzyme called trans-Sialidase (TS), that captures SA from the host and transfers it to Mucins, also membrane proteins, which store SA. This study aims at characterizing the nanoscale distribution of TS and Mucins by two-color STORM (Stochastic Optical Reconstruction Microscopy)