PERSONAL DE APOYO
LOPEZ Lucia Fernanda
artículos
Título:
Pulsed Interleaved MINFLUX
Autor/es:
MASULLO, LUCIANO A.; STEINER, FLORIAN; ZÄHRINGER, JONAS; LOPEZ, LUCIÁ F.; BOHLEN, JOHANN; RICHTER, LARS; COLE, FIONA; TINNEFELD, PHILIP; STEFANI, FERNANDO D.
Revista:
NANO LETTERS (PRINT)
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2021 vol. 21 p. 840 - 846
ISSN:
1530-6984
Resumen:
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.