INVESTIGADORES
CABRERIZO Franco Martin
congresos y reuniones científicas
Título:
DNA damage induced by photoexcited bare and loaded Microporous Coordination Polymers
Autor/es:
MATÍAS RAFTI; JUAN G. YAÑUK; M. MICAELA GONZALEZ; FRANCO M. CABRERIZO
Reunión:
Encuentro; Fourth Latin American Meeting on Biological Inorganic Chemistry (IV LABIC). Fifth Workshop on Bioinorganic Chemistry (IV WOQUIBIO); 2014
Resumen:
Microporous Coordination Polymers (MCPs also known as Metal Organic Frameworks or MOFs) are a relatively new class of organic-inorganic hybrid crystalline solids constituted by metal ions/clusters and organic linkers forming a porous network. Because of the great variety of possible metal ions and linkers, MCPs are a highly tuneable material in terms of pore dimension and shape, and surface functional groups present. This leads to a wide range of possible applications such as, gas and liquid phase adsorptive separations and purifications, membrane based separations, catalysis, drug delivery, and energy-relevant gas storage. b-carbolines (bCs) are a family of alkaloids widely found in nature highly active from a biological point of view. They have shown activity against several microorganism (bactaria, parasites, viruses, etc.). Upon excitation, the antimicrobial activity of these molecules is considerably increased. The photosensitizing properties of bCs and some related derivatives have been recently well studied and documented. On cell-free DNA, photoexcited harmine induces single strand-breaks (SSB), 8-oxo-7,8-dihydroguanine and pyrimidine dimers formation (CPDs). In the present work, we report on the capability of both bare and harmine-loaded MCPs to photoinduce damage in cell-free DNA. The MCPs chosen were two comercially available Cu and Fe based materials (BASF Baseolite C300 and F300 respectively, both featuring the organic linker benzene tricarboxylic acid, BTC). The extent of the damage is analyzed together with the physicochemical and photochemical properties of the investigated compounds.