INVESTIGADORES
DENOFRIO Maria Paula
congresos y reuniones científicas
Título:
3,4-Dihydro-bC;-carboline alkaloids as potential endogenous photosensitizers
Autor/es:
FERNANDO D. VILLARRUEL; FRANCO M. CABRERIZO; ROSA ERRA-BALSELLS; M. PAULA DENOFRIO
Reunión:
Encuentro; XIII Encuentro Latinoamericano de Fotoquímica y Fotobiología (ELAFOT); 2017
Resumen:
β-carbolines (βCs) represent a family of naturally occurring alkaloids and their presence has been confirmed in a vast range of phylogenetically distant species, i.e., Rhizaria, Alveolata, Amoebozoa, Stramenopiles, Opisthokonta, Archaeplastida, Urochordata and Arthropoda. From the chemical point of view, βCs comprise both full-aromatic and partially hydrogenated (such as 3,4-dihydro- and 1,2,3,4-tetrahydro-) heterocyclic structures. In particular, harmaline and harmalol are the most representative and naturally distributed dihydro-βC derivatives. In terms of its chemical structure, harmine and harmol represent the corresponding full-aromatic structures (Scheme 1).During the last decade, the photochemistry and photodynamic properties of some full-aromatic βCs have been deeply studied. It has been well documented that these properties strongly depend on the chemical nature of the βC substituent [1-6]. However, the information available in the literature regarding the chemical properties of photo-excited 3,4-dihydro- and 1,2,3,4-tetrahydro-βCs, is still negligible or null and, when available, is somehow contradictory [7].In the present work, we have investigated the photochemical behavior of two particular 3,4-dihydro-βCs, harmaline and harmalol, in aqueous solutions under different O2 concentrations. Also, we examine the capability of these βCs to photoinduce damage in cell-free DNA. Results obtained are compared with data recorded for the corresponding full-aromatic βC, harmine and harmol respectively