INVESTIGADORES
GLISONI Romina Julieta
congresos y reuniones científicas
Título:
SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES AS AN HYBRID (RADIOACTIVE-FLUORESCENCENT) IMAGING AGENT
Autor/es:
SIMOIS S.; CAMMARATA A.; GLISONI RJ.; ARAUJO J.; POGGI M.; GAMBINI JP.; CABRAL P.
Lugar:
Mar del Plata
Reunión:
Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9 Participan AACYTAL . NANOMED-AR . HCS; 2019
Institución organizadora:
SAIC-NANOMED-ar
Resumen:
Introduction. In the last decades, nanotechnology has radically changed the diagnosis of many human pathologies. Particularly, silver nanoparticles (AgNPs) have provided powerful biomedical applications in different areas and effectively contributed to the diagnosis of oncological diseases. The aim of this work was to obtain AgNPs with appropriate properties for hybrid (radioactive-fluorescent) (99mTc-AgNPs-ICG) imaging. Methods. For the preparation of AgNPs, three different solutions were prepared: (i) silver nitrate 2.5x10-3 M solution, (ii) ascorbic acid 1.7x10-4 M solution and (iii) citric acid 8.0x10-4 M solution. Briefly, 2 mL of (ii) were mixed with 5 mCi of 99mTcO4-, subsequently 2 mL of (iii) and 0.5 mL of (i), permanently stirring for 20 minutes at 37 °C. Preparation final pH was 5. Finally, 2µL of green indocyanine (ICG, 1.3 mM) was added to obtained 99mTc-AgNPs-ICG. A TOSOHAAS 6300SWXL HPLC column (7.8mmx30cm), double-distilled water as mobile phase (flow of 1 mL/min) and an UV detector (λ1 = 420 nm and λ2 = 254 nm) and gamma detector were used to characterize 99mTc-AgNPs-ICG. Fluorescence imaging was performed using BRUKER MS FX PRO equipment. Nanoparticle size, size distribution and polidispersity indexes (PDI) was analyze by dynamic light scattering (DLS) and FT-IR spectrum, were determined. Results. AgNPs was obtain in aqueous solution and remained stable for at least one week. HPLC profile shown a good correlation between both the UV and GAMA chromatogram, with matching retention times. Main peak spectrum presented an absorption band close to 420 nm, corresponding to plasmon absorption band for AgNPs. The hydrodynamic diameter was 46 ± 1.5 nm and the FT-IR spectrum displayed characteristic bands at 3193, 2624, 1596 and 1212 cm-1, confirming the synthesis. Conclusion. Stable silver nanoparticles with hybrid (radioactive-fluorescent) dual labeling (99mTc-AgNPs-ICG) were obtain with a simple and reproducible method. These silver nanoparticles could potentially be used as scintigraphic and fluorescent imaging agents for different biomedical applications. Acknowledgement ANII, CSIC, PEDECIBA for financial support.