INVESTIGADORES
GLISONI Romina Julieta
congresos y reuniones científicas
Título:
HYBRID LIPOSOMES FOR SENTINEL NODE
Autor/es:
CABRERA M.; GONZÁLEZ C.; GLISONI RJ.; TASSANO M.; FERNANDEZ LOMONACO M.; GAMBINI JP.; CABRAL P.
Lugar:
Mar del Plata
Reunión:
Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9 Participan AACYTAL . NANOMED-AR . HCS; 2019
Institución organizadora:
SAIC-NANOMED-ar
Resumen:
Introduction. Intraoperative lymphatic mapping and sentinel node biopsy (BGC) followed by selective complete lymphadenectomy, is a strategy for the treatment of patients with solid tumors that determines a greater sensitivity in histopathological evaluation and lymph node staging and a lower surgical morbidity. The nanoparticles are absorbed by the lymphatic system and be retained in the regional lymph nodes at the injection site. Among its advantages are high stability, high load capacity, possibility of incorporating hydrophilic and hydrophobic substances. The objective of this work was to develop a new hybrid radiopharmaceutical using 99mTc-labeled nanoparticles and fluorescein. Methods: Liposomes were prepared by ?hand-shaken? method, obtaining DTPA-liposome film combining phosphatidylcholine, cholesterol and stearylamide-DTPA in chloroform mixture: methanol (2: 1 v/v), rotated to dryness in a water bath. 60 ° C It was hydrated with fluorescein, DMSO and double distilled water, obtaining multilamellar vesicles extruded by 400 nm membranes. Nanoparticle size, size distribution and polidispersity indexes (PDI) was analyze by dynamic light scattering (DLS). These were labeled with 99mTc, chemical controls were performed by ascending chromatography and molecular exclusion. In vitro stability was assessed by confronting both complexes at different concentrations of cysteine. As a preclinical evaluation, normal C57 black mice injected into a hind leg were used subcutaneously. Images were taken at 5, 10, 15, 30 and 60 minutes post-injection. Results: The size of the liposomes was (410 ± 40) nm. The labeling yield was> 90%. Biodistribution in normal mice at 1 hour post-injection for 99mTc-DTPA-Liposomes-fluorescein demonstrates statistically significant uptake in the lymph nodes of the injected member compared to the lymph nodes of the non-injected member (0.40% ± 0.04, expressed as the average of% Act / gr ± DS vs 0.04% ± 0.01%, p = 0.02. Conclusion: The hybrid tracers tested in this work could be a radiopharmaceutical potential for lymph node evaluation in the sentinel node technique. Acknowledgement ANII, CSIC, PEDECIBA for financial support.