ENDOPLASMIC RETICULUM STRESS MODULATES CELLULAR SENESCENCE IN ENDOMETRIAL STROMAL CELLS

FERNÁNDEZ L; GORI S; BESTACH Y; GRASSO E; GALLINO L; SOCZEWSKI E; IRIGOYEN M; MARTINEZ G; PÉREZ LEIRÓS C; RAMHORST R

Congreso; Reunión Anual de Sociedades de Biociencias; 2021

Objectives: Endometrial stromal cells undergo an acute senescence response as well as endoplasmic reticulum stress (ERS) during decidual differentiation. Here, we focus on the link between both processes to support embryo implantation.Materials and methods: Human endometrial stromal cell line (HESC) was decidualized with MPA and db-cAMP for 8 days (Dec). To induce ERS, non-decidualized (nonDec) cells were stimulated with 1ug/ml of thapsigargin (Tg) for 4h. Public data of genome-wide transcriptome analysis of primary ESCs (GSE160702) was used to evaluate expression of senescence-associated genes Deiodinase 2 (DIO2), Lumican (LUM), Ferritin (FTL) and Forkhead box protein O1 (FOXO1). Gene expression was evaluated by RT-qPCR in HESC cells and in endometrial biopsies from fertile women and recurrent implantation failure (RIF) patients. FOXO1 and β-galactosidase activity were tested by FACS. *p