THE DECIDUALIZATION INDUCED BY VIP ACTIVATES RETICULAR STRESS AND UNFOLDED PROTEIN RESPONSE AND CONDITIONS ENDOMETRIAL RECEPTIVITY

ELIZABETH VICTORIA SOCZEWSKI; MARIA SOLEDAD GORI; ESTEBAN GRASSO; LAURA FERNÁNDEZ; LUCILA GALLINO; MARCELA IRIGOYEN; THALITA F. LOBO; ROSIANE MATTAR; SILVIA DAHER; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Congreso; Reunión conjunta SAIC-SAI-SAFIS 2018; 2018

The vasoactive intestinal peptide (VIP) induces the decidualization program and conditions the immunoregulation of the implantation process. Endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), which will allow them to induce a physiological sterile inflammatory response through inflammasome activation and IL-1beta production. Here, we focus on VIP effect on the RS and UPR pathways induced by the decidualization process and whether modulates inflammation.We used an in vitro model of human decidualization represented by human endometrial stromal cell line (HESC) differentiated with VIP (10-7M) or with medroxyprogesterone (MPA 10-6M) and dibutyryl-cAMP (2,5 10-3M) during 8 days.VIP significantly increased the expression of ATF6, a RS-sensor, and CHOP, an UPR marker (p