THE INFLAMMATORY RESPONSE DURING THE IMPLANTATION PERIOD: ASSOCIATION WITH THE RETICULAR STRESS AND THE UNFOLDED PROTEIN RESPONSE

ELIZABETH VICTORIA SOCZEWSKI; MARÍA SOLEDAD GORI; ESTEBAN GRASSO; LAURA FERNÁNDEZ; THALITA FRUTUOSO; SILVIA DAHER; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Embryo implantation in humans involves the generation of a sterile inflammatory response associated with blastocyst invasion; however, it is still unclear how it could be induced. During decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR) which allow them to expand their endoplasmic reticulum and change their secretome producing proimplantatory mediators. Here we focus on the impact of the RS/UPR on decidual cells and how could be induced IL-1b production needed for implantation. We used two approaches: an in vitrodecidualization model of Human endometrial stromal cell line (Dec) differentiated with MPA (1uM) and dbcAMP (2,5mM); and endometrial biopsies obtained from fertile women, with recurrent spontaneous abortions (RSA) or with in vitro fertilization failures (IF).The decidualization in Dec cells increased the RS-sensors, ATF6, PERK and IRE1a in comparison with non-Dec cells (Thapsigargin was used as ctrol+ p