HISTAMINE RECEPTORS H3/H4 INVOLVEMENT IN A MOUSE MODEL OF DERMATITIS

ALCAIN J; GORI S; SALAMONE G; VERMEULEN M

Mar del Plata

Congreso; Reunión Conjunta SAIC-SAI-SAFE 2016; 2016

&ermatitis is an inflammatory disease characterized by an imbalance of the immune response toYard a Th profile, Yhose main inflammatory mediator is histamine H+S. H+S exerts its actions through four receptors HH. 2reviously, Ye demonstrated, using a model of pulmonary inflammation to 18A, that H+S is able to induce the recruitment of C& T lymphocytes in the lung of mice. The purpose of the present YorM Yas to study the modulatory role of H+S in another inflammatory disease affecting the sMin, contact dermatitis, and the role of H and H receptors. For this, Ye developed a mouse model of dermatitis using the irritating agent dinitrophenyl benzene . &0F$ in acetone/Yater. For this, Çl of &0F$ or vehicle Yas administered for consecutive days on both ears, along Yith Çl of a H/H receptor antagonist (thioperamide, Thio, 10-7M) on the right ear. Seven days after the first administration, &0F$ . in 2$S Yas administered intraperitoneally in all mice. Topical treatment of the injured zone Yith Thio partially reversed the symptoms of the disease associated Yith inflammation in the application area Ct, . mm v . mm &0F$, . mm v . mm &0F$Thio, . mm v . mm. p ., n, +F0º and +L production by T lymphocytes, Yithouth affecting T cell proliferation in a mixed allogenic reaction. +nterestingly, the application of Thio at the site of injury stimulated C&b C&c cell recruitment Ct, . v . Thio, . v . &0F$, . v . &0F$Thio . v . p., n and inhibited C&c C&b cell recruitment Ct, . v . Thio, . v . &0F$, . v . &0F$Thio, . v .. p . in the zonal lymph node. +n conclusion, these results could indicate that the use of an antagonist of H/H receptors could reverse the effects induced in an inflammatory disease such as dermatitis through the inhibition of both Th response and epithelial sYelling.