IMMUNE HOMEOSTASIS DURING THE DECIDUALIZATION PROGRAM: PARTICIPATION OF VIP AND DENDRITIC CELLS

GRASSO E; GORI S; PAPARINI D; GALLINO L; SOCZEWSKI E; SALAMONE G; PÉREZ LEIRÓS C; RAMHORST R

Mar del Plata

Congreso; VI SLIMP and V LASRA-Latin American Society for Maternal Fetal Interaction and Placenta; 2015

SLIMP-LASRA

The decidualization program involves phenotype and functional changesin endometrial cells such as the production of different mediators that willfacilitate the attachment and invasion of the blastocyst. Particularly, thevasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrialstromal cells among others, which displays multiple target circuitsthat allow immunotolerance.Objective: We investigated VIP contribution to the decidualization programand whether this process modulates the profile of dendritic cells(DC).Methods: A human endometrial stromal cell line (hESC) was evaluated.These cells were differentiated in the presence of VIP (10-7M) ormedroxyprogesterone (MPA) and dbcAMP (Positive control) for 8 days.Phenotypic markers of decidualization, VIP and their receptors werequantified by RT-PCR. DC were obtained from monocytes isolated fromPBMCs from fertile women and differentiated with IL-4 and GM-CSF. VIPand IL-10 secretion were quantified by ELISA.Results: When decidualization was induced by VIP, we observed anincrease in characteristic markers such as IGFBP1, PRL and the KLF13/KLF9 ratio, IL-8 and SDF1 in a concentration-dependent manner(p