ALTERATIONS IN ENDOMETRIAL PROGRAMMING LEADS TO RECURRENT IN VITRO FERTILIZATION FAILURES

LARA CASTAGNOLA; SOLEDAD GORI; ELIZABETH SOCZEWSKI; ESTEBAN GRASSO; LAURA FERNÁNDEZ; ANA SCHAFIR; MARCELA IRIGOYEN; GUSTAVO MARTÍNEZ; LUCILA GALLINO; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC-SAI 2022; 2022

SAI-SAIC-SAFIS

Embryo implantation efficiency in humans is very low because it requires a competent blastocyst during the endometrium’s window ofimplantation (WOI). Patients with repeated implantation failure (RIF)don’t reach implantation even if a competent blastocyst is transferredduring WOI. Our hypothesis is that RIF patients present alterationson the decidualization program, affecting decidual cells’ functions,and on the associated inflammatory response. First, we performedbioinformatic analysis in endometrial biopsies from RIF patients vsfertile, based on standardized pathways in public data bases. Wefocused on genes that connected and were modulated in processesinvolved in implantation, angiogenesis, placentation, decidualization, inflammation and immune regulation. Then, we validated expression of 15 genes with highest score in biopsies from RIF or fertilewomen taken on LH+7 by RT-qPCR. We found decreased IGFBP1expression, early decidualization marker, and the progesteronereceptor (p