EXTRACELLULAR VESICLES FROM THE PERIODONTAL BACTERIA PORPHYROMONAS GINGIVALIS REDUCE GLUCOSE METABOLISM IN TROPHOBLAST CELLS MODIFYING THEIR ANTI-INFLAMMATORY AND TOLEROGENIC EFFECTS

BRENDA LARA; IÑAKI LOUREIRO; LAURA GLIOSCA; GUILLERMINA CALO; SOLEDAD GORI; ANA SCHAFIR; FLORENCIA SABBIONE; ANALÍA TREVANI; ROSANNA RAMHORST; DAIANA VOTA; CLAUDIA PÉREZ LEIRÓS; VANESA HAUK

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC-SAI 2022; 2022

SAI-SAIC-SAFIS

Adverse pregnancy outcome and deep placentation disorders areassociated with periodontal disease. Porphyromonas gingivalis(Pg), one of the main pathogens causing periodontitis, releasesextracellular vesicles known as outer membrane vesicles (PgOMV)that play an important role in the host-pathogen interaction. During placentation, trophoblast cells (Tb) secrete soluble factors in a closeinteraction with maternal leukocytes to maintain immune homeostasis. An impaired trophoblast-immune interaction is associated withpregnancy complications. Previously we reported that PgOMV diminished the migration and invasion capacity of Tb. Our aim wasto study the effect of PgOMV on Tb cell metabolism and trophoblast-immune interaction. Human first trimester trophoblast cell lineHTR-8 was stimulated with 1 µg/ml PgOMV for 4-24h. Lipid droplets, glucose and fatty acid uptake were analyzed by flow cytometryusing Bodipy 493/503, 2-NBDG and FLC12 probes, respectively.Lactate production was measured by Accutrend Plus system andmRNA expression of inflammatory mediators by qPCR. Peripheralblood monocytes and neutrophils were purified from healthy donorsand cultured with conditioned media from Tb (CM) pre-treated ornot with Pg-OMV (PgOMV-CM). Reactive oxygen species (ROS)production was evaluated by flow cytometry. PgOMV treatment reduced glucose uptake and the release of lactate by trophoblast cells(Basal 8.3±0.8 mM; Pg-OMV 7.2±0.4 mM; X±SEM n=4 p50% decrease inIL-8 (p