Cellular senescence association to endoplasmic reticulum stress in endometrial stromal cells

LAURA FERNÁNDEZ; MARÍA SOLEDAD GORI; YÉSICA BESTACH; ESTEBAN GRASSO; LUCILA GALLINO; ELIZABETH SOCZEWSKI; MARCELA IRIGOYEN; GUSTAVO MARTÍNEZ; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

Congreso; IX SLIMP 2022; 2022

Objectives:During the decidualization process, endometrial stromal cells (EnSCs) undergo endoplasmic reticulum stress (ERS). Recently, it was reported that a subpopulation of EnSCs also undergoes cellular senescence. Here, we hypothesize there is a link between both processes.Methods:Human endometrial stromal cell line (HESC) was in vitro decidualized with MPA and db-cAMP for 8 days (Dec). To induce ERS, nondecidualized (nonDec) cells were stimulated with 1ug/ml thapsigargin (Tg), a strong pharmacological ERS inductor. Endometrial samples were obtained from fertile women and recurrent implantation failure (RIF) patients. Additionally, public data of genome-wide transcriptome analysis of primary EnSCs (GSE160702) was used to evaluate gene expression in primary cultures from human EnSCs. RT-qPCR was used to evaluate expression levels of senescence markers: Deiodinase 2 (DIO2) and Lumican (LUM); as well as mature Dec cells markers: Ferritin (FTL) and Forkhead box protein O1 (FOXO1). FOXO1 andb-galactosidase activity weretested by FACS. *p