Trophoblast cells induce a tolerogenic profile in dendritic cells

GABRIELA SALAMONE; LAURA FRACCAROLI; SOLEDAD GORI; ESTEBAN GRASSO; DANIEL PAPARINI; JORGE GEFFNER; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST

HUMAN REPRODUCTION

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2012 vol. 27 p. 2598 - 2606

0268-1161

Background: Dendritic cells (DCs), which are biased toward a tolerogenic profile, play a pivotal role in tissue-remodeling processes and angiogenesis at the maternal?fetal interface. Here, we analyzed the effect of trophoblast cells on the functional profile of DCs to gain insight on the tolerogenic mechanisms underlying the human placental?maternal dialog at early stages of gestation. Methods: DCs were differentiated from peripheral blood monocytes obtained from fertile women (n=21), in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor during 5 days in culture. Then, DCs were cultured with trophoblast cells (Swan-71 cell line obtained from normal cytotrophoblast, at 7 weeks) for 24 h and for an additional 24 h in the absence or presence of lipopolysaccharide (LPS) from Escherichia coli. DCs were recovered and used for flow cytometry, enzyme-linked immunosorbent assay, RT PCR and suppression and migration assays. Results: Trophoblast cells significantly prevented the increase in CD83 expression induced by LPS without affecting the expression of CD86, CD40 and human leukocyte antigen-DR (P