MODULATION OF DENDRITIC CELL APOPTOSIS AND CD8+ CYTOTOXICITY BY HISTAMINE: ROLE OF PROTEIN KINASE C.

ALCAIN J; PODAZA E; GORI MS; SALAMONE G; VERMEULEN M

MEDIATORS OF INFLAMMATION

HINDAWI PUBLISHING CORPORATION

Lugar: New York; Año: 2017

0962-9351

Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC. In fact, HIS is involved in the recruitment of specific CD8 + lymphocytes during the development of chronic inflammatory responses. In this study, we showed that the cytotoxicity of specific CD8 + lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a G q subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated that HIS prevents the apoptosis of DC after heat shock at 42°C, a mechanism dependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS stimulated DC was independent of PKC pathway.