PERSONAL DE APOYO
ADROVER Ezequiela
congresos y reuniones científicas
Título:
Prenatal stress effect on glutamate metabolism, evaluated with mass spectrometry- liquid chromatography
Autor/es:
EZEQUIELA ADROVER; MARÍA ROSA KATUNAR; HELLE S. WAAGEPETERSEN; ARNE SCHOUSBOE; M C ANTONELLI
Lugar:
Busan, Corea del norter
Reunión:
Congreso; 22nd Biennial Meeting of the Internacional Society for Neurochemistry; 2009
Institución organizadora:
Internacional Society for Neurochemistry (ISN)
Resumen:
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Prenatal
stress effect on glutamate metabolism, evaluated with liquid cromatography-mass
spectrometry.
Ezequiela Adrover1,
María Rosa Katunar1,
Helle S. Waagepetersen2,
Arne Schousboe2 y Marta
C. Antonelli1. 1IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquímica. UBA. Buenos Aires. Argentina. 2Faculty of Pharmaceutical SciencesUniversity of Copenhagen. Copenhagen. Denmark.
Episodes of stress
suffered by the mother during pregnancy generate changes in the fetal
environment affecting central nervous system development of the offspring. Previous studies from our laboratory
have shown that adult offspring of rats stressed during the last week of
pregnancy exhibited higher levels of glutamate receptors than control rats
concomitantly with a long-lasting
astroglial reaction and a reduced dendritic arborization with synaptic loss in
the brain of adult offsprings. Since metabolism of glutamate and GABA is
intimately linked to a substrate cycle between neurons and sorrounding
astroglia, our results prompted us to suggest that both glutamate and GABA
neurotransmitter pathways might be impaired in the brain of a prenatally
stressed rat. To study the effect of prenatal stress (PS) on the
metabolism of glutamate, pregnant rats were subjected to restrain stress during
the last week of gestation. Brain extracts of PS rats were evaluated by liquid
chromatography and mass spectroscopy to measure the content of glutamate, GABA
and others metabolites. This procedure was performed in frontal cortex and
caudate putamen brain extracts of animals of 28 and 60 postnatal day (PND). Our
results show that glutamate content in adult male (PND 60) PS rats is
significantly reduced than in control rats. This would indicate that prenatal
stress produces long-term changes in the metabolism and/or synthesis of
glutamate thereby modulating the expression of glutamate receptors and altering
the normal synaptic transmission of the adult brain.