PERSONAL DE APOYO
ADROVER Ezequiela
congresos y reuniones científicas
Título:
Dopaminergic neurotransmission in a model of prenatal stress in rats
Autor/es:
MR. KATUNAR, E. ADROVER, VG. BARROS, A. SILVAGNI, A. BRUSCO, E. CARBONI Y MC. ANTONELL
Lugar:
Washington, DC., EEUU
Reunión:
Congreso; 38th Anual Meeting of the Society for Neuroscience; 2008
Institución organizadora:
Society for Neuroscience (SFN)
Resumen:
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Dopaminergic
neurotransmission in a model of prenatal stress in rats
M.R. Katunar1, E. Adrover1 , V.G. Barros1, A. Silvagni3, A. Brusco2, E.
Carboni3, M.C. Antonelli1.
1IQUIFIB, Facultad de Farmacia y Bioquímica,UBA. 2 IBCyN
Prof. Eduardo De Robertis, Facultad de Medicina, UBA. Buenos Aires, ARGENTINA.
3 Dipartimento de Tossicologia, Università Degli Studi di Cagliari, Cagliari,
ITALY
*e-mail:
mkatunar@qb.ffyb.uba.ar
We have previously shown that prenatal
stress (PS) increases D2-type dopamine (DA) receptor (D2R) levels in frontal
cortex, nucleus accumbens (NAcc) core and hippocampus. In this study, we found
that prenatal stress increased D2R both in the left and right sides of NAcc
core, but the left-right asymmetry observed in the control group, was selectively
lost in adult rats exposed to prenatal stress. In addition, we measured the
levels of DA in brain by microdialysis following amphetamine injections to
freely moving rats. We observed a decrease of amphetamine stimulated dialysate
in prefrontal cortex of prenatally stressed rats of 8 weeks old as compared
with controls. Employing an inmunocytochemistry approach, we studied the
expression levels of two transcription factors Nurr1 and Pitx3 which are
expressed at critical moments of DA neurons differentiation. We found a
ubiquitous distribution of Nurr-1
in cerebral cortex, hippocampus, thalamus, amygdala and
midbrain whereas Pitx3 remains restricted to the mesencephalic DA system such
as substancia nigra (SN) and ventral tegmental area (VTA). Our results show
that the expression of both Nurr-1 and Pitx3 increased in prenatally stressed
adult offspring in the VTA area, whereas no changes were observed in SN areas.
Taken together these results show that several components of the limbic
dopaminergic system are disregulated as a consequence of the prenatal insult.
Loss of asymmetries of the D2 receptors in NacC can lead to long term
behavioral and cognitive impairments. The increase of the specific dopaminergic
transcription factors might be a compensatory mechanism to counteract the
reduction in dopamine levels observed in PFC, which in turn might be
upregulating D2 dopaminergic receptors.