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Glutamate metabolism in a model of prenatal restraint stress in rats
ADROVER E; PALLARÉS ME; BAIER CJ; MONTELEONE MC; BROCCO MA; HELLE S. WAAGEPETERSEN; ARNE SCHOUSBOE; ANTONELLI MC
Congreso; 41st Annual Meeting of the Society for Neuroscience 2011 (SFN; 2011
Rats exposed to different types of stress during gestation have shown severe anomalies in neural development and brain morphology that persist into adulthood. Previous studies from our laboratory demonstrated that prenatally stressed rats exhibited higher levels of glutamate receptors than control (C) rats. These animals also show long-lasting astroglial reaction and a reduced dendritic arborization with synaptic loss. Since glutamate metabolism is linked to a cycle between neurons and sorrounding astroglia, our results suggest that glutamate neurotransmitter pathways might be impaired in the brain of these animals. To study the effect of prenatal stress (PS) on the glutamatergic system, pregnant rats were subjected to restrain stress during the last week of gestation and brain extracts from adult offpring were evaluated. We study the glutamate transporters uptake, protein and mRNA levels in prefrontal cotex and hippocampus of 60 days old male and female rats. Our results show that PS male rats have a significantly higher glutamate uptake when compared with C subjects. We also found elevated mRNA and protein levels of glutamate transporters. This would indicate that prenatal stress produces long-term changes in the glutamatergic system modulating the expression of glutamate receptors and altering the normal synaptic transmission of the adult brain.