PERSONAL DE APOYO
congresos y reuniones científicas
¨Gestational stress: A morphological and endocrine overview in male rat progeny¨
PALLARÉS, M.E1.; ADROVER, E1; KATUNAR M.R.1; BAIER C.J.1; CALANDRA R.S. 2, GONZÁLEZ-CALVAR S.I.2; ANTONELLI M.C1
Congreso; 40th Anual Meeting of the Society for Neuroscience (SFN); 2010
Maternal stress has been reported to induce long term behavioral and biochemical abnormalities in their offspring. For example, it was shown to suppress testosterone (T) surge that occurs in the male embryo between gestational days 17 and 18, responsible for the brain sexual differentiation. In a first approach to evaluate the hypothalamic- pituitary- testicular (HPT) axis in our model of prenatal stress (PS) we analyzed external and histological morphometric parameters and quantified androgens serum levels. Pregnant Wistar dams were subjected to three daily 45 min period sessions of restraint stress, between 14 and 21 days of gestation. Anogenital distances were measured at postnatal day (PND) 1, 10 and 21. Analysis of testis descent, defined as the day when both testes fully descended into the scrotal sac, was initiated on PND 21. Hormonal status of 28, 35, 45, 60 and 75 days old male progeny was determined by measuring T and 5-a Androstane- 3-a, 17b- Diol (DIOL) serum levels by radioimmunoassay. Finally, the testes of 35 and 60 days old animals were processed for histological morphometric measures: the maturation of the spermatogenic epithelium was assessed by applying the Johnsens score. We also quantified the mean tubular diameter and Leydig cells number. PS reduced anogenital distance of the progeny at PND 21 in comparison to control (C) animals. Moreover, while C animals complete their testis descent at PND 23, PS animals showed a 2-day delay (i.e. PND 25). T serum levels analysis revealed a 40% increase on PS offspring T serum concentrations in comparison with C at PND 60 and 75. Diol serum levels were found to increase 61 and 63% in PS offspring at PND 28 and 45 respectively. Additionally, we found an accelerated spermatogenesis on 35 and 60 days old PS rats showing a higher Johnsens score, in comparison to C animals. The mean tubular diameter was also augmented in PS animals. However, the mean Leydig cells number was reduced on PS rats. Our results show a reduced anogenital distance and delayed testicular descent in the offsprings. Additionally, the accelerated maturation of the spermatogenesis observed in PS offspring, might be related to the increase of androgen serum levels at earlier stages of life. In summary, our studies demonstrate that stress during gestation induces long term effects on the male progeny HPT axis and testicular morphology.