PERSONAL DE APOYO
ADROVER Ezequiela
congresos y reuniones científicas
Título:
EFFECTS OF PRENATAL STRESS ON MALE RAT HYPOTHALAMIC- PITUITARY- TESTICULAR AXIS
Autor/es:
PALLARÉS M.E1.; GONZÁLEZ-CALVAR S.I.2; BOURGUIGNON N.S.3; ADROVER E1; KATUNAR M.R.1; BAIER C.J.1; LUX-LANTOS V.3;CALANDRA R.S.2; ANTONELLI M.C1
Reunión:
Congreso; V Congreso de la Sociedad de Neurotoxicidad (NTS); 2011
Resumen:
We tested the hypothesis that prenatal exposure to
restraint stress (PS) alters hypothalamic-pituitary-testicular (HPT) axis
status in male offspring as a possible effector of previously observed
dopaminergic (DA) metabolism impairments. Luteinizing hormone (LH), Follicle
stimulating hormone (FSH), Testosterone (T) and 5-a Androstane- 3-a, 17b- Diol
(DIOL) serum levels of 28, 35, 45, 60 and 75 days old male progeny were
determined by radioimmunoassay. Testes of 35 and 60 days old animals were
processed for androgen receptor (AR) quantification by western blot and for
histological morphometric measures. Hormones serum levels analyses revealed
that PS diminished LH levels at post natal day (PND) 28 and 75 in comparison with control
group (C). In PS group, FSH levels were decreased at PND 28 while T serum
levels were reduced at 75 days old rats. However, PS increased DIOL levels at
PND 28 and 45. Furthermore, the rate of spermatogenesis was accelerated on PS
rats and the mean tubular diameter was increased. On the other hand, the mean
Leydig cells number was reduced on PS rats. Finally, we did not find
statistical changes between groups on AR levels in testis measured by western
blot. Our results are in agreement with the hypothesis that prenatal stress
alters offspring HPT. Our future challenge is to elucidate the consequences of
the hormone milieu imbalance on DA metabolism impairments.