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EFFECTS OF PRENATAL STRESS ON MALE RAT HYPOTHALAMIC- PITUITARY- TESTICULAR AXIS
PALLARÉS M.E1.; GONZÁLEZ-CALVAR S.I.2; BOURGUIGNON N.S.3; ADROVER E1; KATUNAR M.R.1; BAIER C.J.1; LUX-LANTOS V.3;CALANDRA R.S.2; ANTONELLI M.C1
Congreso; V Congreso de la Sociedad de Neurotoxicidad (NTS); 2011
We tested the hypothesis that prenatal exposure to restraint stress (PS) alters hypothalamic-pituitary-testicular (HPT) axis status in male offspring as a possible effector of previously observed dopaminergic (DA) metabolism impairments. Luteinizing hormone (LH), Follicle stimulating hormone (FSH), Testosterone (T) and 5-a Androstane- 3-a, 17b- Diol (DIOL) serum levels of 28, 35, 45, 60 and 75 days old male progeny were determined by radioimmunoassay. Testes of 35 and 60 days old animals were processed for androgen receptor (AR) quantification by western blot and for histological morphometric measures. Hormones serum levels analyses revealed that PS diminished LH levels at post natal day (PND) 28 and 75 in comparison with control group (C). In PS group, FSH levels were decreased at PND 28 while T serum levels were reduced at 75 days old rats. However, PS increased DIOL levels at PND 28 and 45. Furthermore, the rate of spermatogenesis was accelerated on PS rats and the mean tubular diameter was increased. On the other hand, the mean Leydig cells number was reduced on PS rats. Finally, we did not find statistical changes between groups on AR levels in testis measured by western blot. Our results are in agreement with the hypothesis that prenatal stress alters offspring HPT. Our future challenge is to elucidate the consequences of the hormone milieu imbalance on DA metabolism impairments.