Cupric Reductase Activity in Copper-Resistant Amycolatopsis tucumanensis

DÁVILA COSTA, JOSÉ SEBASTIÁN; ALBARRACÍN VIRGINIA HELENA; ABATE CARLOS MAURICIO

WATER AIR AND SOIL POLLUTION

SPRINGER

Lugar: Netherlands; Año: 2011 vol. 216 p. 527 - 535

0049-6979

Amycolatopsis tucumanensis, a recently recognized novel species showed remarkable copper resistance as well as efficient Specific Cupric Reductase Activity (SRACu) in both, copper adapted and non adapted cells, under different temperatures of incubation. Its copper resistance strength was highlighted against other metal resistant actinobacteria (Streptomyces sp. AB5A) and sensitive strains (Amycolatopsis eurytherma and Streptomyces coelicolor). Pre-adapted cells of A. tucumanensis displayed values of SRACu, on average, 65% higher than those obtained from non-adapted cells. In addition, preadaptation of A. tucumanensis improved the rate of Cu(II) reduction which was approximately, 2-fold, 7-fold and 9-fold higher than pre-adapted cells from other Streptomyces sp. AB5A, A. eurytherma and S. coelicolor, respectively. A. tucumanensis showed the highest levels of SRACu at all temperatures and also the higher copper resistance profile, suggesting that these two abilities may be in close relationship. This ostensible versatility, related to the temperature, of adapted cells from A. tucumanensis might support the application of this strain under different bioremediation conditions. To our knowledge this is the first time that cupric-reductase activity was demonstrated within the genus Amycolatopsis.