congresos y reuniones científicas
EVOLUTIONARY ANALYSES OF INDETERMINATE DOMAIN (IDD) TRANSCRIPTION FACTORS IN ANGIOSPERMS
PROCHETTO S.; REINHEIMER R.
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
The indeterminate domain (IDD) transcription factors are a subclassof C2H2 Zinc Finger transcription factor family. This transcriptionfactors plays different roles in diverse aspects of plant metabolismand development like, flowering time, seed maturation, rootdevelopment, shoot gravitropism and leaf development. The functionsof most of these genes are not known and the molecular evolutionof the family has not been explored in detailed.Here we present a phylogenetic tree constructed by Bayesian inferenceusing the IDD genes of 10 representative genomes of floweringplants (Angiosperms) The protein sequences were obtainedof the GreenPhylDB and Phytozome databases, they were alignedwith MAFFT program, and conserved blocks were extracted andused to make a phylogenetic reconstruction using Mr Bayes v3.2.6.We also performed a conserved motif analyses using MEME v4.11.Our results suggest that the family may be divided in at least ninegroups. These lineages share three conserved motives, which includethe four zinc fingers of the IDD domain. Additionally, we foundmotives that are group specific that needs further characterization.Furthermore, four of the nine groups seem to be monocots specificIDD lineages. Interestingly one of these monocots groups includesIndeterminate1 that controls transitioning to flowering in Zea maysand is the gene for which the family is named.This study provides a solid framework of the orthology relationshipsin the Angiosperm IDD transcription factors, thus increasingthe accuracy of ortholog identification in model species and facilitatingthe identification of agronomically important genes related toplant metabolism and development.