INVESTIGADORES
COCERES Veronica Mabel
congresos y reuniones científicas
Título:
Biogenesis of extracellular vesicles released by the parasite Trichomonas vaginalis
Autor/es:
SALAS, N; COCERES VM; DE MIGUEL, N
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencia 2019; 2019
Resumen:
T. vaginalisis a parasite that causes trichomoniasis, the most common non-viral sexuallydisease worldwide. Given it is an extracellular parasite, adhesion to hostcells is one of the key processes for the development of infection. In recentyears, various factors that influence this process have been identified,including extracellular vesicles (EVs). Previously in our laboratory we demonstratedthat both exosomes (vesicles with a size range of 40-100 nm) and ectosomes(vesicles with a size of 100 - 1000 nm) are involved in the process ofinteraction of T.vaginalis with host cells. It is currently knownthat ESCRT-III subunit of to the ESCRT complex is involved in membrane cleavagebeing the VPS32 protein the key effector during membrane scission. These antecedentslead to our hypothesis that VPS32 might be regulating the process ofextracellular vesicles formation in T. vaginalis. Based on this, we transfectedthe protein VPS32 and an empty vector as a control (EpNEO) in a poorly-adherent parasite strain and evaluated the amount of EVs released by theseparasites by TEM, NTA and western blot using Evs markers.Our results demonstrated that VPS32 transfected parasites released more EVsthan EpNEO parasites. The amount of both EVs populations is affected;suggesting that VPS32 is involved in the biogenesis of exosomes as well asectosomes. As our previous results demonstrated that EVs are regulatingparasites adhesion and now, we observe that VPS32 parasites produces more EVs,we performed an adhesion assay to host cells to evaluate the adherence capacityof VPS32 and EpNEO parasites. Interestingly, our results indicate thatparasites transfected with VPS32 are ~40 times more adherents than EpNEOparasites. In summary, our results demonstrated that VPS32 is a key player inthe regulation of the adherence process; provably due to its role in the increasedbiogenesis of extracellular vesicles.