INVESTIGADORES
MARTINEZ PASTUR Guillermo Jose
artículos
Título:
In vitro propagation of Nothofagus obliqua (Fagaceae).
Autor/es:
G MARTÍNEZ PASTUR; M ARENA
Revista:
AUSTRALIAN JOURNAL OF BOTANY
Referencias:
Año: 1995 p. 601 - 607
ISSN:
0067-1924
Resumen:
Suitable culture conditions were investigated for in vitro propagation through shoot culture of seedlings and the acclimatization of the rooted plantlets of Nothofagus obliqua.
Explant size and leaf expansion were critical for culture initiation. A
medium with a low salt concentration and the addition of L-glutamine,
as broadleaved tree medium, was suitable for shoot multiplication and
it was influenced by both cytokinin and auxin. A multiplication rate of
x5 has been obtained with 0.55 micro M benzyladenine (BA), and 0.09
micro M BA and 0.09 micro M IBA at day 63. Rooted shoots (51.9%) were
obtained with 0.61 IBA and 1 week darkness at the induction stage.
Rooted plants were then successfully acclimatised and 75% survival rate
was obtained after 4 months.
