INVESTIGADORES
MARTINEZ PASTUR Guillermo Jose
artículos
Título:
Adventitious shoot induction from leaf explants of Ribes magellanicum cultured in vitro.
Autor/es:
M ARENA; G MARTÍNEZ PASTUR
Revista:
SCIENTIA HORTICULTURAE
Editorial:
Elsevier
Referencias:
Año: 1997 p. 73 - 79
ISSN:
0304-4238
Resumen:
Several factors were studied on adventitious shoot induction from leaf
explants of Ribes magellanicum cultured in vitro: type of explant,
position of the leaves along the stem and growth regulators. Explants
with petiole and complete leaf lamina presented the highest percentage
of organogenesis. The position of the leaf along the shoot strongly
affected the induction capacity. The percentage of explants with
adventitious shoots was maximum (53%) when using apical leaves, with an
average of three shoots per explant. 6-benzylaminopurine (BA) and
N-benzyl-9-(2-tetrahydropyranyl)-adenine (BPA) had a significantly
higher promotive effect than 6-(τ-τ-dimethylallylamino) purine (2iP) or
kinetin on induction of adventitious shoots. Cytokinin concentration
significantly affected shoot production, best responses being obtained
with 4.44 to 8.88 μM. No significant differences were observed between
α-naphthaleneacetic acid (NAA) concentrations but 0.5 μM NAA usually
promoted the highest number of non-vitrified shoots per explant. The
results of this work show that R. magellanicum can be induced to form
adventitious shoots from leaf explants cultured in vitro.