Micropropagación de Nothofagus pumilio (Poepp et Endl) Krasser.

G MARTÍNEZ PASTUR; M ARENA

BOSQUE (VALDIVIA)

Año: 1997 p. 43 - 50

0304-8799

Nothofagus pumilio is the most important forest species in South Patagonia. The present work was carried out todetermine the suitable cultural conditions for in vitro propagation through shoot culture of saplings and a maturetree. The days after sprouting and the presence of the bud scales were critical for culture initiation. A medium witha low salt concentration and the addition of glutamine, as Broadleaved Tree Medium, was suitable for shootmultiplication, being affected both by cytokinin and giberellin. A multiplication rate of 1:3-5 was obtained with 20-30 μM 2iP and 0.3 μM GA3 at day 63. No significant differences were found between the juvenile and adultmaterial. Rooted shoots (60-70%) were obtained with 0.61-1.23 μM AIB and ten days darkness at the inductionstage. Rooted shoots were then successfully acclimatised and 43.2% of good quality plants were obtained after 45days.