6-Benzylaminopurine and activated charcoal affect in vitro shoot morphogenesis of Berberis buxifolia Lam.

M ARENA; G MARTÍNEZ PASTUR

Revista Facultad de Agronomía UBA

Universidad de Buenos Aires

Año: 2001 p. 41 - 47

0325-9250

An important factor affecting the micropropagation is the quality and rate of shoot multiplication.The objectives of this work are to study the effect of concentration and BA continuous presence, andthe addition of activated charcoal during two periods of culture on Berberis buxifolia morphogenesisprocesses during in vitro shoot multiplication. The addition of BA in the three subcultures favored themultiplication index compared with a pulse of BA in the first subculture, specially for 0.55 and 1.10μM BA. Lower BA concentrations (0.55 to 1.10 μM) were more favorable for shoot multiplication than2.22 μM BA, allowing to obtain the highest multiplication index. The percentage of shoots longer than5 mm was maximum with activated charcoal while the multiplication index were significantly lowerthan those obtained without activated charcoal. In the first culture period, the multiplication index werehigher than in the second culture period. However, the percentage of shoots longer than 5 mm wassignificantly higher in the second culture period than in the first culture. The results obtained in thiswork show that the morphological processes observed during in vitro shoot multiplication of B.buxifolia can be controlled by the concentration and continuous presence of BA and the addition ofactivated charcoal.