Changes in isoperoxidase patterns during the in vitro rooting of Nothofagus antarctica.

G MARTÍNEZ PASTUR; D ZAPPACOSTA; M ARENA; N CURVETTO

Bulgarian Journal of Plant Physiology

Año: 2001 p. 43 - 53

1310-4586

Peroxidases play a major role in several physiological processes.Specifically, in vitro activity of this enzyme was used as a marker to identifydifferent rooting phases of Nothofagus. The study of peroxidase profile patternsduring rhizogenesis in this genus could give additional informationabout this topic, to justify the use of peroxidase isoforms as a more specificbiochemical marker than total peroxidase activity. This work gives informationon qualitative and quantitative changes in isoperoxidase expression(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshootsthrough native PAGE. Time course analysis of isoperoxidase activityduring rhizogenesis allowed positive correlation with specific morphogenicstages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Fourof the acidic peroxidases appeared during the induction stage of rooting. Variationin the number and relative activity of the isoperoxidases was found duringdifferent stages of adventitious rooting. The variation in the number andactivity of isoperoxidases provides a good indicator of the processes involvedin adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidaseswith auxin metabolism and the role of certain isoperoxidases in specificrooting processes are also discussed.