INVESTIGADORES
HAMER Mariana
congresos y reuniones científicas
Título:
Development of an electrophoretic method based on nanostructured materials for HbA1c determination
Autor/es:
HAMER MARIANA; NARELLA BASSI; DENISE A. GRELLA
Lugar:
Mendoza
Reunión:
Congreso; 24th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2018
Resumen:
AbstractGlycosylated hemoglobin (HbA1c) detection is performed routinely in hospitals as it is the most widespread confirmatory diagnosis of diabetes mellitus. However, they are not specific for Hb1Ac, the values obtained are relative and quantification of total hemoglobin is additionally necessary. We present a novel CE method for measuring HbA1c by introducing silica nanoparticles (NPs) modified with a boronic acid derivative as pseudo-stationary phase.1 Before the sample injection, SiO2NP-B(OH)2 were introduced via pressure. Electrophoretic separation was explored through variation of the buffer pH and separation voltage, being the best separation, resolution and shorter separation time achieved with a 25mM phosphate buffer pH 6.5. The calibration curve obtained using a UV/VIS absorbance detector at 415 nm (diode array). No interferences were observed from carbamylated or acetylated hemoglobin and the method shows a noteworthy stability. A paired t-test was applied to compare the developed CE method with a commercial HbA1c test and no significant variations have been observed at a 90% significance level. The method shows remarkable stability and can be applied to different glycoprotein or molecule separating purposes with diols forming cyclic boronate esters with organized low energy structures.2References1. Hamer, M.; Bassi, N.; Agata Grela, D. Electrophoresis 2018, 39, 1048?1053.2. Fujita, N.; Shinkai, S.; James, T. D. Chem. ? An Asian J. 2008, 3, 1076?1091.