INVESTIGADORES
HAMER Mariana
congresos y reuniones científicas
Título:
Capillary Electrophoresis Analysis of Organometalic Nanostructures
Autor/es:
HAMER MARIANA; ANGEL YONE; IRENE REZZANO
Reunión:
Simposio; 18th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2012
Resumen:
A method of Capillary Zone Electrophoresis to quantify the ratio of Cu(II) to Fe(III) in self-assembled porphyrin nanostructures was developed. The metalloporphyrins have been used in many technological applications due to their unique physicochemical properties. The interesting optical behavior is significantly amplified in the nanoscopic scale making them versatile sensing materials. In this frame we have prepared different types of nanorods including Fe(III) and Cu(II) metalloporphyrins. The relative amount of Cu(II) to Fe(III) in the nanorods of porphyrins was obtained by Capillary Electrophoresis analysis after exhaustive oxidation with a potassium permanganate saturated solution. The residue was then treated with H2O2: H2SO4 to reduce the manganese oxide and the ionic metals. The solvent was evaporated and the residue dissolved in 25 mM EDTA solution. Standards were equally treated to ensure a good identification. The background electrolyte (BGE) consisted of 20 mM potassium phosphate, pH 8.0. Analyte solutions were introduced in 5 s at 0.5 psi, and the separation voltage was 12 kV. The linear regression equation for Cu(II) system is expressed as y= 361 ppm−1 x - 1.43, R2: 0.9928. The sensitivity for Cu(II) detection is 361ppm (LoQ = 1.77ppm; LoD=0.53 ppm). The linear regression equation for Fe(III) system is expressed as y= 358 ppm−1 x - 637, R2: 0.9974. The sensitivity for Fe(III) detection is 358ppm (LoQ = 1.56ppm; LoD=0.47 ppm). We found out that the proportion of Fe-nanorod was 0.4 and Cu-nanorod 0.6.