AuNPs-coated capillaries for protein and peptide analysis on open-tubular capillary electrochromatography.

HAMER MARIANA; ANGEL YONE; IRENE N. REZZANO

Trieste

Conferencia; Conference on Nanotechnology for Biological and Biomedical Applications (Nano-Bio-Med); 2011

The use of gold nanoparticles in electrophoretic systems to improve the efficiency of separation and the selectivity between selected solutes is described. We present an application of coated capillaries on open tubular electrochromatography (OT-CEC), based on the use of gold nanoparticles (AuNPs) as molecular recognition phase peptide and protein. The immobilization of AuNPs on the capillary wall was performed in a very simple and fast way without requiring heating. The surface features of an AuNPs-coated capillary column were determined using scanning electron microscopy. The chromatographic properties of AuNPs-coated capillaries were investigated through variation of the buffer pH and separation voltage. Effective separations of synthetic peptides mixture were obtained on the AuNPs- coated capillaries. During the optimization process we evaluated the stability of the AuNPs-coated capillary column, without loss of AuNPs. The capacity factor was duplicated. Therefore this modification is stable and can be applied with several separation purposes. A complex mixture of tryptic peptide fragments of HSA was analyzed in both the bare and the AuNPs-coated capillaries. Better electrophoretic peptide profile was observed when using the AuNPs-coated capillary.