INVESTIGADORES
CUESTAS Maria Lujan
artículos
Título:
Silybin-conjugated gold nanoparticles for antimicrobial chemotherapy against Gram-negative bacteria.
Autor/es:
ISLAN GA; DAS S; CACICEDO ML; HALDER A; MUKHERJEE A; CUESTAS ML; ROY P; MUKHERJEE A; CASTRO GR
Revista:
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Editorial:
EDITIONS SANTE
Referencias:
Año: 2019
ISSN:
1773-2247
Resumen:
Gold nanoparticles (AuNPs) have emerged as an interesting tool with high potential in the medical field and as apromising alternative against multidrug-resistant microorganisms. Green chemical synthesis of AuNPs conjugated with the flavonoid silybin (S) to generate a nano-system with dual antibacterial and antioxidant properties were performed. The S-AuNPs plasmon peak was found at 567 nm with narrow particle size distribution ofaround 58 nm. TEM and AFM images revealed the presence of spherical and homogeneous nanoparticles with anaverage diameter of 35 nm. Conjugation of silybin to the surface of AuNPs was confirmed by FTIR analysis.Antioxidant properties were found since the S-AuNPs were able to prevent the rapid oxidation of Brilliant CresylBlue, as reporter dye, in presence of sodium hypochlorite as strong oxidant. Also, S-AuNPs inactivated Gramnegative bacteria of nosocomial interest such as Pseudomonas aeruginosa and Escherichia coli. A minimum inhibitory concentration of 11.2 and 5.6 μg/ml was observed for each microbe respectively. Fluorescent live/deadassay confirmed that S-AuNPs can kill bacteria with significant cell damage only after 30 min of exposition. TEMimages showed that S-AuNPs can strongly interact with bacteria surfaces leading to cell lysis. Also, S-AuNPsshowed about half of cytotoxic concentration (CC50) compared within acyclovir (control) tested in Vero cells.