ACUÑA Carlos Alberto
congresos y reuniones científicas
ANALYSIS OF EXPRESSED SEQUENCES TO CHARACTERIZE THE XENIA EFFECT ON SEEDS OF PASPALUM NOTATUM
FLORENCIA POZZI; GUILLERMO PRATTA; CARLOS A. ACUÑA; SILVINA FELITTI
Congreso; 9TH INTERNATIONAL HERBAGE SEED CONFERENCE; 2017
INTERNATIONAL HERBAGE SEED GROUP
In a previous transcriptomic study about xenia in Paspalum notatum Flüggé, an unpollinated control mother plant was compared to two crosses with pollen donor distinction at ploidy level and mode of reproduction. Considering these results, the objectives of the present work are: 1- Analyze and assign a biological function to the sequences obtained from the transcriptomic analysis of crossings involving paternal genotypes with different level of ploidy and mode of reproduction. 2- Evaluate functions of the sequences during the development of the seed and check if this effect depends on the pollen genotype. cDNA-AFLP analysis from control mother plant ovaries (4x, apomictic pseudogamic) and from crosses with different pollen donor (2x, sexual and 4x, apomictic pseudogamic) was performed three hours after pollination. Thirtythree single differential expression bands were found for the analyzed crosses, 29 of them were sequenced, and assigned a possible biological function by blastn, blastx and blastp searches. The functional characterization of sequences showed that 4 of them (CG3, CG5, GA4 and GA6) presented homology with A. thaliana loci directly associated with embryo sac development, pollen tube development, double fertilization forming a zygote and endosperm. In addition to that, mutant phenotypes of A. thaliana for these loci present a decrease in the size of the siliques, seeds and seed set. These results show evidence of the xenia effect on the functions associated with reproduction in P. notatum. Also, they demonstrate that the genotype of the pollen grain is one of the factors determining the expression of different transcripts that could be related to grain yield.