INVESTIGADORES
CHAVES Analia Silvina
congresos y reuniones científicas
Título:
Effect of yoguhrt administration on recurrent intestinal inflammation model
Autor/es:
CHAVES, S., PERDIGÓN, G., DE MORENO DE LEBLANC, A
Lugar:
Tucumám
Reunión:
Simposio; III Simposio Internacional de II Bacterias Lácticas, Segundo Encuentro Red BAL Argentina; 2009
Institución organizadora:
Centro de Referencias para Lactobacilos
Resumen:
Inflammatory bowel disease may result from exaggerated stimulation of the mucosal immune system by luminal bacterial biota. It was reported the efficacy of the yogurt against IBD but their mechanisms of action are unknown. In previous studies we demonstrated that continuous administration of yoghurt was able to regulate the inflammatory response in a acute intestinal inflammation model induced by 2,4,6 trinitrobenzene sulfonic acid (TNBS). In the present study, we investigated the effect of the yoghurt administration to mice with TNBS-induced recurrent colitis. After 7–9 days, mice inoculated with TNBS that recovered their initial weight were divided into two groups. One group received yoghurt ad libitum during 21 days, and the other group received water and conventional food ad libitum, at the end of feeding period the samples were taken. The remaining mice of each group received a 2nd intrarectal administration of TNBS. The large intestine and liver were removed 3d after the 2ndad libitum during 21 days, and the other group received water and conventional food ad libitum, at the end of feeding period the samples were taken. The remaining mice of each group received a 2nd intrarectal administration of TNBS. The large intestine and liver were removed 3d after the 2ndad libitum, at the end of feeding period the samples were taken. The remaining mice of each group received a 2nd intrarectal administration of TNBS. The large intestine and liver were removed 3d after the 2ndnd intrarectal administration of TNBS. The large intestine and liver were removed 3d after the 2ndnd TNBS administration to evaluate liver translocation, colon histology and intestinal microbiota. Weight changes and mortality were also evaluated. The recurrent control group showed the highest weight loss 2 and 3 days after the 2° TNBS administration compared with the mice that received yoghurt administration in the same periods of time (21.37±0.97 y 20.72±1.95 for TNBS-TNBS group; 28.15±2.36, 27.743±3.13 for TNBS-Y-TNBS group, for 2 and 3 days, respectively). The mortality rate shown significantly differences compared the recurrent control group (3-8%) with the mice given yoghurt (without mortality). The control recurrent group showed the most important changes in the structure of large intestine and the highest score of colonic damage (5.3±0.7). Mice fed 21 days with yoghurt showed a significant increase in the bifidobacteria counts (5.28±0.14 CFU) 3 days after of 2° TNBS administration, compared to the control recurrent group without yoghurt (2.01 ± 0.2 CFU) Enterobacteria population diminished in mice that received yoghurt (3.37±0.93 CFU) compared with control recurrent group (6.70±1.35 CFU). For other bacterial population, differences were not observed. LT did not show differences between control and yoghurt feeding group, 21days after 1° TNBS administration. TNBSTNBS group increased the percentage of mice with liver translocation evaluated in the three different media (MacConkey, LAPTg and MRS). Mice from TNBS-Y-TNBS group showed lower percentage of translocation than the TNBS-TNBS group. We demonstrated that yoghurt administration was able to attenuate the symptoms of recurrent intestinal inflammation, and reduced the liver translocation, probably by desirable changes observed in the intestinal microbiota.