INVESTIGADORES
MARTORELL Maria Martha
congresos y reuniones científicas
Título:
SCREENING OF DECOLOURIZING YEASTS WITH DYE ASSIMILATION ABILITY FROM LAS YUNGAS RAINFOREST
Autor/es:
MARTORELL, MARIA M.; PAJOT, HIPOLITO F.; FARINA, JULIA I.; FIGUEROA, LUCIA I. C.
Lugar:
Puerto Madryn
Reunión:
Congreso; SAIB 2010; 2010
Resumen:
Environmental pollution by textile-dye containing wastewaters is a matter  of  worldwide  concern.  Yeasts  bioremediation,  a  poorly explored  and  less  exploited  methodology,  stands  out  as  an interesting  approach  supported  on  their metabolic  diversity  and high growth rates. In this work, a screening protocol based on dye tolerance  and  dye  assimilation  ability  on  solid  media  was performed with a 2000 mg/L dye mixture. Thirty-nine yeast  isolates from  “Laurel  del Monte”  underlying  soils  from  “Las Yungas” (Tucumán, Argentina) were  obtained. Based  on  decolourization haloes  and  colony  dyeing,  15  isolates  showed  the  highest decolourization ability on agar plates containing Vilmafix® Blue RR-BB,  Vilmafix®  Red  7B-HE,  Vilmafix®  Black  B-V  and Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening on agar plates with synthetic media supplemented with each dye as sole C or N source, led to the selection of 10 isolates. Microsatellite RAPD analysis enabled the discrimination of these isolates into six groups. Following  tests consisted on  the analysis of growth and decolourization  kinetic  profiles,  along  with  the  production  of Manganese  peroxidase,  laccase  and  tyrosinase  activities  in  dye- supplemented  (200  mg/L)  liquid  media.  Time-dependent supernatant spectra, growth curves and enzymatic activities led to the  selection  of  the  two  most  promising  isolates  for  future experiments.