INVESTIGADORES
MARTORELL Maria Martha
congresos y reuniones científicas
Título:
SCREENING OF DECOLOURIZING YEASTS WITH DYE ASSIMILATION ABILITY FROM LAS YUNGAS RAINFOREST
Autor/es:
MARTORELL, MARIA M.; PAJOT, HIPOLITO F.; FARINA, JULIA I.; FIGUEROA, LUCIA I. C.
Lugar:
Puerto Madryn
Reunión:
Congreso; SAIB 2010; 2010
Resumen:
Environmental pollution by textile-dye containing wastewaters is a
matter of worldwide concern. Yeasts
bioremediation, a poorly
explored and less exploited methodology,
stands out as an
interesting approach supported on their metabolic
diversity and
high growth rates. In this work, a screening protocol based on dye
tolerance and dye assimilation ability on
solid media was
performed with a 2000 mg/L dye mixture. Thirty-nine yeast isolates
from Laurel del Monte underlying soils
from Las Yungas
(Tucumán, Argentina) were obtained. Based
on decolourization
haloes and colony dyeing, 15 isolates
showed the highest
decolourization ability on agar plates containing Vilmafix® Blue
RR-BB, Vilmafix® Red 7B-HE, Vilmafix® Black
B-V and
Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening
on agar plates with synthetic media supplemented with each dye as
sole C or N source, led to the selection of 10 isolates. Microsatellite
RAPD analysis enabled the discrimination of these isolates into six
groups. Following tests consisted on the analysis of growth and
decolourization kinetic profiles, along with
the production of
Manganese peroxidase, laccase and tyrosinase
activities in dye-
supplemented (200 mg/L) liquid media. Time-dependent
supernatant spectra, growth curves and enzymatic activities led to
the selection of the two most
promising isolates for future
experiments.