Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents

MARTORELL, MARIA M.; FERNANDEZ, PABLO M.; FARINA, JULIA I.; FIGUEROA, LUCIA I. C.

INTERNATIONAL BIODETERIORATION & BIODEGRADATION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2012 vol. 71 p. 80 - 85

0964-8305

Chromium-resistant yeasts isolated from contaminated environments can be used to reduce toxic Cr(VI). This study assessed in vitro reduction of hexavalent chromium using crude chromate reductase (CChR) of Pichia jadinii M9 and Pichia anomala M10, two yeasts isolated from a textile-dye factory effluent. CChRs were characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. Both CChRs showed an increase in Cr(VI) reductase activity with addition of NAD(P)H as electron donor and were highly inhibited by Hg2+ and Mn2+. The CChR from P. jadinii M9 showed the highest chromate reductase activity at 60 °C and pH 6.0 in the presence of Cu2+ or Na+, while P. anomala M10 CChR had the maximum activity at 50 °C and pH 7.0 in presence of Cu2+. Initial Cr(VI) concentrations of 1.3 and 1.7 mM for CChRs of P. jadinii M9 and P. anomala M10 respectively were inhibitory.