INVESTIGADORES
ADLER Conrado
congresos y reuniones científicas
Título:
THE SbmA LOCUS IS INVOLVED IN THE SWARMING MOTILITY AND BIOFILM FORMATION
Autor/es:
CORBALAN NATALIA; POMARES FERNANDA; DE CRISTOBAL RE; ADLER CONRADO; MONICA DELGADO; VINCENT, PAULA
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; SAIB; 2010
Institución organizadora:
SAIB
Resumen:
SbmA protein is involved in the transport of MccB17, MccJ25,bleomycin and proline rich peptides into the E. coli cytoplasm.Although SbmA homologues were found in a variety of bacteria, thephysiological role of this protein is unknown. The SbmAhomologous BacA from Brucella abortus and Sinorhizobiummeliloti is essential for the chronic infection of these pathogens. Theswarming and biofilm formation are two important bacterialmulticellular behaviors whereby the pathogen bacteria enhancedtheir chances of survival in competitive environments and infect thehost. In this work we evaluated the possible importance of sbmAgene in biofilm formation and swarming motility. We observed thatthe sbmA absence produced a significant increase in both behaviors.On the other hand, we note that the mutation in the gene yaiw, whichintegrates an operon together sbmA , was not able to affect biofilmand swarming. Curiously, the inductor effect of the sbmA mutationwas reverted when tolC or trpE mutation were added. Since in mostpathogens the biofilm is often related to virulence, the capacity ofsbmA Salmonella mutant to infect and replicate inside ofmacrophages was evaluate. The sbmA mutant showed an increasedreplication ability comparing with the wild-type after 48 hours.Taken together these results suggest an important physiological roleof sbmA in the virulence phenotype