INVESTIGADORES
ESPASANDIN Fabiana Daniela
artículos
Título:
Putrescine accumulation in Arabidopsis thaliana transgenic lines enhances tolerance to dehydration and freezing stress
Autor/es:
ANALÍA I. ALET, DIEGO H. SANCHEZ, JUAN C. CUEVAS, SECUNDINO DEL VALLE, TERESA ALTABELLA, ANTONIO F. TIBURCIO, FRANCISCO MARCO, ALEJANDRO FERRANDO, FABIANA D. ESPASANDÍN, MARÍA E. GONZÁLEZ, PEDRO CARRASCO, OSCAR A. RUIZ
Revista:
Plant Signalling and Behavior
Editorial:
Landes biosciences
Referencias:
Lugar: Bonn; Año: 2010 vol. 6 p. 181 - 189
ISSN:
1559-2324
Resumen:
Polyamines have been globally associated to plant responses to abiotic stress. Particularly, putrescine has been related
to a better response to cold and dehydration stresses. It is known that this polyamine is involved in cold tolerance, since
Arabidopsis thaliana plants mutated in the key enzyme responsible for putrescine synthesis (arginine decarboxilase, ADC;
EC 4.1.1.19) are more sensitive than the wild type to this stress. Although it is speculated that the overexpression of ADC
genes may confer tolerance, this is hampered by pleiotropic effects arising from the constitutive expression of enzymes
from the polyamine metabolism. Here, we present our work using A. thaliana transgenic plants harboring the ADC gene
from oat under the control of a stress-inducible promoter (pRD29A) instead of a constitutive promoter. The transgenic
lines presented in this work were more resistant to both cold and dehydration stresses, associated with a concomitant
increment in endogenous putrescine levels under stress. Furthermore, the increment in putrescine upon cold treatment
correlates with the induction of known stress-responsive genes, and suggests that putrescine may be directly or indirectly
involved in ABA metabolism and gene expression.