INVESTIGADORES
PIECKENSTAIN Fernando Luis
congresos y reuniones científicas
Título:
Polyamine metabolism of tobacco plants infected by Sclerotinia sclerotiorum
Autor/es:
MARINA M, E RIVAS, A GÁRRIZ, MC DALMASSO, FL PIECKENSTAIN, OA RUIZ
Lugar:
Bariloche, Argentina
Reunión:
Congreso; XXXIX Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas; 2003
Institución organizadora:
Sociedad Argentina de Investigaciones Bioquímicas
Resumen:
Polyamines are polycationic compounds that are found in all living organisms. In plants, they have been shown to be essential for growth and developmental processes, and recently, a defense– related role was suggested. Regarding this, high levels of spermine were observed in tobacco plants after TMV infection and spermine itself was able to induce the expression of pathogenesis-related proteins in a salicylic-independent way. Furthermore, an increase of polyamines in plants infected by biotrophic fungi has been reported but information about nechrotrophic fungi is lacking This work was aimed to evaluate the role of polyamines in tobacco plants in response to the infection of the nechrotrophic fungus Sclerotinia sclerotiorum, the most important pathogen of cultivated sunflower in Argentina. Tobacco plants inoculated with S. sclerotiorum mycelium showed higher levels of putrescine than controls in whole tobacco leaves, but when intercellular spaces was studied considerable changes in putrescine, spermidine and spermine was observed. In order to evaluate the specific role of each polyamine, we infiltrated tobacco leaf discs with solutions of these compounds before the inoculation. In this case, polyamine infiltration resulted in an increase of necrotic area of inoculated leaf discs, suggesting that either polyamines are deleterious to defense response of tobacco plants or they are able to support fungal development. The probable role of polyamines in tobacco- S. sclerotiorum interaction will be discussed.