INVESTIGADORES
OTERO Maria Claudia
congresos y reuniones científicas
Título:
Vaginal flora modifications during the growth of healthy cows
Autor/es:
OTERO, MARIA CLAUDIA; SAAVEDRA, LUCILA; SILVA, CLARA; PESCE DE RUIZ HOLGADO, AÍDA; NADER-MACÍAS, MARIA ELENA
Lugar:
Los Angeles. EE.UU.
Reunión:
Otro; General Meeting American Society for Microbiology; 2000
Institución organizadora:
American Society for Microbiology
Resumen:
Vaginal flora modifications during the growth of healthy cows    A stable microbial ecosystem in the vaginal tract acts as an important defense mechanisms against pathogenic microorganisms. Perturbations of this stability may allow the onset of infections, some of them causing metritis, which produces delayed periods from the partum to the conception, and the consequent economical losses. In the present work we studied the normal microbial flora of vaginal cows and their evolution from the weanning to the service. Our interest is to know which are the dominant group of microorganisms, which are the first capable of colonizing and which dominate over the time to study their application as probiotic microorganisms.Vaginal swab samples were taken from 15 heifers every two months during two years. Selective and enriched culture media were used to isolate and quantitate the microorganisms. They were identified by phenotypic characteristics. Our results show that the total aerobic flora are between 104-105 CFU/sample. The dominant group is Enterococci, following Staphylococci, whith similar levels during all the period studied (102-104 CFU/sample). The group of Enterobacteriaceae and Lactobacilli showed very low levels (100-102 CFU/sample) in the first period, increasing lactobacilli while the cows were growing suggesting some kind of hormonal influence. The present work will allow the selection of those microorganisms classified as GRAS, based on their probiotic characteristics, to be used in the prevention of infections of the vaginal tract of cows.